Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| nuclear receptor subfamily 4, group A, member 2 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Nurr1 recombinant protein (Position: D383-F598). Human Nurr1 shares 100% amino acid (aa) sequence identity with both mouse and rat Nurr1. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NR4A2 |
Protein |
Nuclear receptor subfamily 4 group A member 2 |
Uniprot ID |
P43354 |
Function |
Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons (By similarity). |
Tissue Specificity |
Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue. |
Sub-cellular localization |
Cytoplasm. Nucleus. Note: Mostly nuclear; oxidative stress promotes cytoplasmic localization. |
Sequence Similarities |
Belongs to the nuclear hormone receptor family. NR4 subfamily. |
Aliases |
HZF 3 antibody|HZF3 antibody|Immediate-early response protein NOT antibody|Intermediate early receptor protein antibody|NGFI B/nur77 beta type transcription factor homolog antibody|NOT antibody|NR4A2 antibody|NR4A2_HUMAN antibody|nuclear receptor of T cells antibody|nuclear receptor related 1 antibody|Nuclear receptor subfamily 4 group A member 2 antibody|Nur related protein 1 homolog antibody|nur related protein-1, human homolog of antibody|Nurr 1 antibody|Orphan nuclear receptor NR4A2 antibody|Orphan nuclear receptor NURR1 antibody|RNR 1 antibody|RNR1 antibody|T cell nuclear receptor NOT antibody|T-cell nuclear receptor NOT antibody|TINUR antibody|Transcriptionally inducible nuclear receptor antibody|Transcriptionally inducible nuclear receptor related antibody|Transcriptionally inducible nuclear receptor related 1 antibody|Transcriptionally-inducible nuclear receptor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Nurr1 antibody, ASA-B1427, Western blotting
All lanes: Anti Nurr1 (ASA-B1427) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: HUT Whole Cell Lysate at 40ug
Predicted bind size: 67KD
Observed bind size: 67KD
All lanes: Anti Nurr1 (ASA-B1427) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: HUT Whole Cell Lysate at 40ug
Predicted bind size: 67KD
Observed bind size: 67KD
