Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| oligodendrocyte transcription factor 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Olig1(94-109aa ARPDAKEEQQQQLRRK), different from the related mouse and rat sequences by four amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
OLIG1 |
Protein |
Oligodendrocyte transcription factor 1 |
Uniprot ID |
Q8TAK6 |
Function |
Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube (By similarity). |
Tissue Specificity |
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas is highly variable. |
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Contains 1 bHLH (basic helix-loop-helix) domain. |
Aliases |
Basic domain helix loop helix protein class B 6 antibody|Basic domain helix loop helix protein class B6 antibody|BHLH B6 antibody|BHLHB 6 antibody|BHLHB6 antibody|bHLHe21 antibody|Class B basic helix-loop-helix protein 6 antibody|Class E basic helix-loop-helix protein 21 antibody|Olig 1 antibody|Olig1 antibody|OLIG1_HUMAN antibody|Oligo 1 antibody|Oligo1 antibody|Oligodendrocyte lineage transcription factor 1 antibody|Oligodendrocyte specific bHLH transcription factor 1 antibody|Oligodendrocyte transcription factor 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- OLIG1 antibody, ASA-B1430, Western blotting
All lanes: Anti OLIG1 (ASA-B1430) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
All lanes: Anti OLIG1 (ASA-B1430) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
