Description
Data Sheet
| Biological Activity | The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 2-10ng/ml. |
| Description | Oncostatin-M Human Recombinant produced in HEK cells is a glycosylated monomer, having a total molecular weight of 30kDa.The OSM is purified by proprietary chromatographic techniques. |
| Expression host | HEK. |
| Formulation | The OSM was lyophilized from 1mg/ml in 1xPBS. |
| Inactivation | UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| Physical Appearence | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Protein Background | Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. |
| Purity | Greater than 95% as obsereved by SDS-PAGE. |
| Solubility | It is recommended to reconstitute the lyophilized OSM in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized OSM although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution OSM should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | OSM, MGC20461. |
