Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cyclin-dependent kinase inhibitor 1B (p27, Kip1) | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human P27 KIP 1 recombinant protein (Position: S10-T198). Human P27 KIP 1 shares 87% amino acid (aa) sequence identity with mouse P27 KIP 1. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CDKN1B |
Protein |
Cyclin-dependent kinase inhibitor 1B |
Uniprot ID |
P46527 |
Function |
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. |
Tissue Specificity |
Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney. |
Sub-cellular localization |
Nucleus. Cytoplasm. Endosome . Note: Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity). |
Sequence Similarities |
Belongs to the CDI family. |
Aliases |
AA408329 antibody|AI843786 antibody|Cdki1b antibody|CDKN 1B antibody|CDKN 4 antibody|CDKN1B antibody|CDKN4 antibody|CDN1B_HUMAN antibody|Cyclin Dependent Kinase Inhibitor 1B antibody|Cyclin dependent kinase inhibitor p27 antibody|Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody|Cyclin-dependent kinase inhibitor 1B antibody|Cyclin-dependent kinase inhibitor p27 antibody|Cyclin-dependent kinase inhibitor p27 Kip1 antibody|KIP 1 antibody|KIP1 antibody|MEN1B antibody|MEN4 antibody|OTTHUMP00000195098 antibody|OTTHUMP00000195099 antibody|p27 antibody|p27 Kip1 antibody|P27-like cyclin-dependent kinase inhibitor antibody|P27KIP1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-P27 KIP 1 antibody, ASA-B1451–1.jpg
All lanes: Anti-p27 KIP 1(ASA-B1451) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
All lanes: Anti-p27 KIP 1(ASA-B1451) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
