Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| purinergic receptor P2X, ligand-gated ion channel, 4 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human P2RX4(368-388aa REKKYKYVEDYEQGLASELDQ). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
P2RX4 |
Protein |
P2X purinoceptor 4 |
Uniprot ID |
Q99571 |
Function |
Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. |
Tissue Specificity |
|
Sub-cellular localization |
Membrane. |
Sequence Similarities |
Belongs to the P2X receptor family. |
Aliases |
P2X4 antibody|P2X4R antibody|ATP receptor antibody|Purinergic receptor antibody|Purinoceptor P2X4 antibody|P2X receptor, subunit 4 antibody|Purinergic receptor P2X, ligand-gated ion channel, 4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- P2RX4 antibody, ASA-B1453, Western blotting
All lanes: Anti P2RX4 (ASA-B1453) at 0.5ug/ml
Lane 1: MCF-7Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 43 KD
Observed bind size: 77KD
All lanes: Anti P2RX4 (ASA-B1453) at 0.5ug/ml
Lane 1: MCF-7Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 43 KD
Observed bind size: 77KD
