Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| purinergic receptor P2X, ligand-gated ion channel, 7 |
Polyclonal |
IgG |
Rabbit |
Human, Mouse, Rat |
WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the N-terminus of rat P2RX7(1-20aa MPACCSWNDVFQYETNKVTR), different from the related mouse sequence by one amino acid, and from the related human sequence by two amino acids. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
P2RX7 |
Protein
|
P2X purinoceptor 7 |
Uniprot ID
|
Q64663 |
Function
|
Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. |
Tissue Specificity
|
|
Sub-cellular localization
|
Cell membrane. |
Sequence Similarities
|
|
Aliases
|
ATP receptor antibody|P2X purinoceptor 7 antibody|P2X7 antibody|P2Z receptor antibody|Purinergic receptor antibody|purinergic receptor P2X, ligand gated ion channel, 7 antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human, Rat Mouse |
AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-P2RX7 antibody, ASA-B1455, Western blotting
WB: U87 Cell Lysate
