Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| proprotein convertase subtilisin/kexin type 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human PC1/3(726-740aa DVFYNTKPYKHRDDR), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PCSK1 |
Protein |
Neuroendocrine convertase 1 |
Uniprot ID |
P29120 |
Function |
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasmic vesicle, secretory vesicle. Note: Localized in the secretion granules. |
Sequence Similarities |
Belongs to the peptidase S8 family. Furin subfamily. |
Aliases |
BDP antibody|EC antibody|NEC 1 antibody|NEC1 antibody|NEC1_HUMAN antibody|Neuroendocrine convertase 1 antibody|PC1 antibody|PC3 antibody|PCSK1 antibody|Prohormone convertase 1 antibody|Prohormone convertase 1/3 antibody|Prohormone convertase 3 antibody|Proprotein convertase 1 antibody|SPC3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PC1/3 antibody, ASA-B1486, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: A549 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: PANC Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: A549 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: PANC Cell Lysate
Anti-PC1/3 antibody, ASA-B1486, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
