PCNA Antibody (monoclonal)

PCNA Antibody (monoclonal)

$330.00

In stock

SKU: ASA-B1489 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
proliferating cell nuclear antigen Monoclonal IgG Mouse Human, Mouse, Rat IHC-P, IHC-F, ICC, WB Ascites

Immunogen
Protein A fusion protein.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 PCNA

Protein

 Proliferating cell nuclear antigen

Uniprot ID

 Human(P12004), Mouse(P17918), Rat(P04961)

Function

 Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase’s processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3′- 5′ exonuclease and 3′-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while ‘Lys-63’-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Tissue Specificity

Sub-cellular localization

 Nucleus.

Sequence Similarities

 Belongs to the PCNA family.

Aliases

 Cyclin|DNA POLYMERASE DELTA AUXILIARY PROTEIN

Application Details

Application Concentration* Species Validated Using**
Western blot 2μg/ml Human, mouse, rat AssaySolutio’s ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.4-1μg/ml Human, mouse, rat AssaySolutio’s IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.4-1μg/ml Human, mouse, rat AssaySolutio’s IHC/ICC Detection kit
Immunocytochemistry Suitable Human, mouse, rat AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-PCNA antibody (monoclonal), ASA-B1489, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 4: COLO320 Cell Lysate
Anti-PCNA antibody (monoclonal), ASA-B1489, IHC(P)
IHC(P): Human Rectal Cancer Tissue
Anti-PCNA antibody (monoclonal), ASA-B1489, ICC
ICC: HELA Cell

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