Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| CD274 molecule | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human PD-L1 recombinant protein (Position: E45-T290). Human PD-L1 shares 69% amino acid (aa) sequence identity with mouse PD-L1. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CD274 |
Protein |
Programmed cell death 1 ligand 1 |
Uniprot ID |
Q9NZQ7 |
Function |
Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. |
Tissue Specificity |
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. |
Sub-cellular localization |
Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities |
Belongs to the immunoglobulin superfamily. BTN/MOG family. |
Aliases |
B7 H antibody|B7 H1 antibody|B7 homolog 1 antibody|B7-H1 antibody|B7H antibody|B7H1 antibody|CD 274 antibody|CD274 antibody|Cd274 antibody|CD274 antigen antibody|CD274 molecule antibody|MGC142294 antibody|MGC142296 antibody|OTTHUMP00000021029 antibody|PD L1 antibody|PD-L1 antibody|PD1L1_HUMAN antibody|PDCD1 ligand 1 antibody|PDCD1L1 antibody|PDCD1LG1 antibody|PDL 1 antibody|PDL1 antibody|Programmed cell death 1 ligand 1 antibody|Programmed death ligand 1 antibody|RGD1566211 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PD-L1 antibody, ASA-B1491–1.jpg
All lanes: Anti PDL1 (ASA-B1491) at 0.5ug/ml
WB: Recombinant Human PDL1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti PDL1 (ASA-B1491) at 0.5ug/ml
WB: Recombinant Human PDL1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti-PD-L1 antibody, ASA-B1491–2.jpg
All lanes: Anti PDL1 (ASA-B1491) at 0.5ug/ml
WB: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
All lanes: Anti PDL1 (ASA-B1491) at 0.5ug/ml
WB: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
