Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| periostin, osteoblast specific factor | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Periostin(811-824aa DTPVRKLQANKKVQ), different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
POSTN |
Protein |
Periostin |
Uniprot ID |
Q15063 |
Function |
Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity). Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. |
Tissue Specificity |
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. |
Sub-cellular localization |
Golgi apparatus . Secreted, extracellular space, extracellular matrix. |
Sequence Similarities |
Contains 1 EMI domain. |
Aliases |
MGC119510 antibody|MGC119511 antibody|OSF 2 antibody|OSF-2 antibody|OSF2 antibody|Osteoblast specific factor 2(fasciclin I like) antibody|osteoblast specific factor 2(fasciclin I-like) antibody|Osteoblast specific factor 2 antibody|Osteoblast specific factor antibody|Osteoblast-specific factor 2 antibody|PDLPOSTN antibody|Periodontal ligament specific periostin antibody|Periostin antibody|Periostin isoform thy2 antibody|Periostin isoform thy4 antibody|Periostin isoform thy6 antibody|Periostin isoform thy8 antibody|Periostin osteoblast specific factor antibody|PN antibody|POSTN antibody|POSTN_HUMAN antibody|RP11 412K4.1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Periostin antibody, ASA-B1500, Western blotting
All lanes: Anti Periostin (ASA-B1500) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 93KD
Observed bind size: 93KD
All lanes: Anti Periostin (ASA-B1500) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 93KD
Observed bind size: 93KD
