Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| peroxiredoxin 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 5(166-181aa DDSLVSIFGNRRLKRF), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PRDX5 |
Protein |
Peroxiredoxin-5, mitochondrial |
Uniprot ID |
P30044 |
Function |
Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. |
Tissue Specificity |
Widely expressed. |
Sub-cellular localization |
Isoform Mitochondrial: Mitochondrion. |
Sequence Similarities |
Belongs to the peroxiredoxin 2 family. |
Aliases |
ACR 1 antibody|ACR1 antibody|Alu co repressor 1 antibody|Alu co-repressor 1 antibody|Alu corepressor 1 antibody|antioxidant enzyme B166 antibody|AOEB166 antibody|B166 antibody|liver tissue 2D-page spot 71B antibody|MGC117264 antibody|MGC142283 antibody|MGC142285 antibody|mitochondrial antibody|Peroxiredoxin 5 mitochondrial antibody|Peroxiredoxin 5, mitochondrial antibody|Peroxiredoxin V antibody|Peroxiredoxin-5 antibody|Peroxiredoxin5 antibody|peroxisomal antioxidant enzyme antibody|PLP antibody|PMP 20 antibody|PMP20 antibody|PRDX 5 antibody|PRDX 6 antibody|PRDX5 antibody|PRDX5_HUMAN antibody|PRDX6 antibody|Prx V antibody|Prx-V antibody|PRXV antibody|SBBI10 antibody|thioredoxin peroxidase PMP20 antibody|Thioredoxin reductase antibody|TPx type VI antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Peroxiredoxin 5 antibody, ASA-B1511, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: 293T Cell Lysate
Lane 7: MCF-7 Cell Lysate
Lane 8: A549 Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: 293T Cell Lysate
Lane 7: MCF-7 Cell Lysate
Lane 8: A549 Cell Lysate
Anti-Peroxiredoxin 5 antibody, ASA-B1511, IHC(P)
IHC(P): Human Prostatic Cancer Tissue
IHC(P): Human Prostatic Cancer Tissue
Anti-Peroxiredoxin 5 antibody, ASA-B1511, IHC(P)
IHC(P): Rat Liver Tissue
IHC(P): Rat Liver Tissue
