Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| platelet factor 4 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human PF4(85-101aa DLQAPLYKKIIKKLLES). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PF4 |
Protein |
Platelet factor 4(PF-4) |
Uniprot ID |
P02776 |
Function |
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the intercrine alpha (chemokine CxC) family. |
Aliases |
C-X-C motif chemokine 4 antibody|Chemokine(C X C motif) ligand 4 antibody|Chemokine(CXC motif) ligand 4 antibody|CXCL 4 antibody|CXCL4 antibody|Iroplact antibody|MGC138298 antibody|Oncostatin A antibody|Oncostatin-A antibody|OncostatinA antibody|PF 4 antibody|PF-4 antibody|PF4 antibody|Platelet factor 4 antibody|PLF4_HUMAN antibody|SCYB 4 antibody|SCYB4 antibody|short form antibody|Small inducible cytokine subfamily member 4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PF4 antibody, ASA-B1514, Western blotting
Lane 1: Recombinant Human CXCL4 Protein 10ng
Lane 2: Recombinant Human CXCL4 Protein 5ng
Lane 3: Recombinant Human CXCL4 Protein 2.5ng
Lane 1: Recombinant Human CXCL4 Protein 10ng
Lane 2: Recombinant Human CXCL4 Protein 5ng
Lane 3: Recombinant Human CXCL4 Protein 2.5ng
Anti-PF4 antibody, ASA-B1514, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
