Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| placental growth factor |
Polyclonal |
IgG |
Rabbit |
Human |
WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the C-terminus of human PLGF(206-221aa REKMKPERCGDAVPRR). |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
PGF |
Protein
|
Placenta growth factor(PlGF) |
Uniprot ID
|
P49763 |
Function
|
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. |
Tissue Specificity
|
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. |
Sub-cellular localization
|
Secreted. Note: The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. |
Sequence Similarities
|
|
Aliases
|
PGF antibody|PGFL antibody|PIGF antibody|Placenta growth factor antibody|placental growth factor antibody|Placental growth factor vascular endothelial growth factor related protein antibody|PlGF 2 antibody|PlGF antibody|PLGF_HUMAN antibody|PlGF2 antibody|Vascular endothelial growth factor related protein antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human |
AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PLGF antibody, ASA-B1544, Western blotting
WB: Human Placenta Tissue Lysate
