Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| paraoxonase 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human PON1(186-201aa FLDPYLQSWEMYLGLA), different from the related mouse and rat sequences by three amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PON1 |
Protein |
Serum paraoxonase/arylesterase 1 |
Uniprot ID |
P27169 |
Function |
Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. |
Tissue Specificity |
Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. |
Sub-cellular localization |
Secreted, extracellular space. |
Sequence Similarities |
Belongs to the paraoxonase family. |
Aliases |
A esterase 1 antibody|A-esterase 1 antibody|Aromatic esterase 1 antibody|Arylesterase 1 antibody|Arylesterase B type antibody|ESA antibody|Esterase A antibody|K 45 antibody|K-45 antibody|MVCD5 antibody|Paraoxonase 1 antibody|Paraoxonase antibody|Paraoxonase B type antibody|Paraoxonase, plasma antibody|Paraoxonase1 antibody|PON 1 antibody|PON antibody|PON1 antibody|PON1_HUMAN antibody|Serum aryldiakylphosphatase antibody|Serum aryldialkylphosphatase 1 antibody|Serum paraoxonase/arylesterase 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PON1 antibody, ASA-B1554, All Western blotting
All lanes: Anti-PON1(ASA-B1554) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Lung Tissue Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 40ug
Lane 4: Rat Testis Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: HEPA Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: JURKAT Whole Cell Lysate at 40ug
Lane 9: SKOV Whole Cell Lysate at 40ug<br
All lanes: Anti-PON1(ASA-B1554) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Lung Tissue Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 40ug
Lane 4: Rat Testis Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: HEPA Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: JURKAT Whole Cell Lysate at 40ug
Lane 9: SKOV Whole Cell Lysate at 40ug<br
