Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| prolactin | Polyclonal | IgG | Rabbit | Mouse | ELISA, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E. coli-derived mouse Prolactin recombinant protein (Position: L30-C226). Mouse Prolactin shares 59.8% and 84.8% amino acid (aa) sequence identity with human and rat Prolactin, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PRL |
Protein |
Prolactin |
Uniprot ID |
P06879 |
Function |
Prolactin acts primarily on the mammary gland by promoting lactation. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted. |
Sequence Similarities |
|
Aliases |
Decidual prolactin antibody|Lactogenic Hormone antibody|Luteotropic Hormone antibody|Mammotropin antibody|PRL antibody|Prolactin antibody|Prolactin precursor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio’s ECL kit |
| ELISA | 0.1-0.5μg/ml | Mouse | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Prolactin antibody, ASA-B1574, Western blotting
All lanes: Anti Prolactin (ASA-B1574) at 0.5ug/ml
WB: Recombinant Mouse Prolactin Protein 0.5ng
Predicted bind size: 10KD
Observed bind size: 10KD
All lanes: Anti Prolactin (ASA-B1574) at 0.5ug/ml
WB: Recombinant Mouse Prolactin Protein 0.5ng
Predicted bind size: 10KD
Observed bind size: 10KD
