Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| prostaglandin E receptor 1(subtype EP1), 42kDa | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Prostaglandin E Receptor EP1(387-404aa AWEASSLRSSRHSGLSHF). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PTGER1 |
Protein |
Prostaglandin E2 receptor EP1 subtype(PGE receptor EP1 subtype/PGE2 receptor EP1 subtype) |
Uniprot ID |
P34995 |
Function |
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(q) proteins which activate a phosphatidylinositol-calcium second messenger system. May play a role as an important modulator of renal function. Implicated the smooth muscle contractile response to PGE2 in various tissues. |
Tissue Specificity |
Abundant in kidney. Lower level expression in lung, skeletal muscle and spleen, lowest expression in testis and not detected in liver brain and heart. |
Sub-cellular localization |
Cell membrane; Multi-pass membrane protein. |
Sequence Similarities |
Belongs to the G-protein coupled receptor 1 family. |
Aliases |
EP1 antibody|PGE receptor EP1 subtype antibody|PGE2 receptor EP1 subtype antibody|Prostaglandin E receptor 1 subtype EP1 42kDa antibody|Prostaglandin E receptor 1 subtype EP1 antibody|Prostaglandin E2 receptor EP1 subtype antibody|Prostanoid EP1 receptor antibody|PTGER1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Prostaglandin E Receptor EP1 antibody, ASA-B1582, Western blotting
All lanes: Anti Prostaglandin E Receptor EP1 (ASA-B1582) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: MM453 Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
All lanes: Anti Prostaglandin E Receptor EP1 (ASA-B1582) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: MM453 Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
