Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| protein C(inactivator of coagulation factors Va and VIIIa) | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Protein C(446-461aa HGHIRDKEAPQKSWAP). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PROC |
Protein |
Vitamin K-dependent protein C |
Uniprot ID |
P04070 |
Function |
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. |
Tissue Specificity |
Plasma; synthesized in the liver. |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the peptidase S1 family. |
Aliases |
Activation peptide antibody|Anticoagulant protein C antibody|APC antibody|Autoprothrombin IIA antibody|Blood coagulation factor XIV antibody|EC 3.4.21.69 antibody|PC antibody|proC antibody|PROC_HUMAN antibody|PROC1 antibody|Protein C(inactivator of coagulation factors Va and VIIIa) antibody|THPH3 antibody|THPH4 antibody|Vitamin K dependent protein C antibody|Vitamin K dependent protein C precursor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Protein C antibody, ASA-B1584, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: HELA Cell Lysate
Lane 1: JURKAT Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: HELA Cell Lysate
Anti-Protein C antibody, ASA-B1584, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
