Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| RAB3A, member RAS oncogene family | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Rab3A (192-220aa DTADPAVTGAKQGPQLSDQQVPPHQDCAC), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
RAB3A |
Protein |
Ras-related protein Rab-3A |
Uniprot ID |
P20336 |
Function |
Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. |
Tissue Specificity |
Specifically expressed in brain. |
Sub-cellular localization |
Cell membrane ; Lipid-anchor ; Cytoplasmic side . |
Sequence Similarities |
|
Aliases |
Rab 3A antibody|RAB 3A member RAS oncogene family antibody|Rab3a antibody|RAB3A member RAS oncogene family antibody|RAB3A_HUMAN antibody|RAS associated protein RAB 3A antibody|RAS associated protein RAB3A antibody|Ras related protein Rab 3A antibody|Ras related protein Rab3A antibody|Ras-related protein Rab-3A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Rab3A antibody, ASA-B1599, Western blotting
All lanes: Anti Rab3A (ASA-B1599) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 25KD
Observed bind size: 25KD
All lanes: Anti Rab3A (ASA-B1599) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 25KD
Observed bind size: 25KD
