Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| RAB9A, member RAS oncogene family | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
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| A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9(178-193aa RSDHLIQTDTVNLHRK), identical to the related rat sequence, and different from the related mouse sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
RAB9A |
Protein |
Ras-related protein Rab-9A |
Uniprot ID |
P51151 |
Function |
Involved in the transport of proteins between the endosomes and the trans Golgi network. |
Tissue Specificity |
|
Sub-cellular localization |
Cell membrane ; Lipid-anchor ; Cytoplasmic side . Endoplasmic reticulum membrane . Golgi apparatus membrane . Late endosome. Cytoplasmic vesicle, phagosome membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasmic vesicle, phagosome. Note: Colocalizes with OSBPL1A at the late endosome. Recruited to phagosomes containing S.aureus or M.tuberculosis. |
Sequence Similarities |
|
Aliases |
2410064E05Rik antibody|AI195561 antibody|DmRab9 antibody|Rab 9 antibody|RAB 9A antibody|RAB9 member RAS oncogene family antibody|RAB9A antibody|RAB9A member RAS oncogene family antibody|RAS ASSOCIATED PROTEIN RAB9 antibody|Ras related protein Rab 9A antibody|Ras-related protein Rab-9A antibody|Sid6061p antibody|Sid99 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Rab9 antibody, ASA-B1603, All Western blotting
All lanes: Anti-RAB9A(ASA-B1603) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: Mouse Brain Tissue Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: PC12 Whole Cell Lysate at 40ug
Lane 5: NIH Whole Cell Lysate at 40ug
Lane 6: A431 Whole Cell Lysate at 40ug
Lane 7: 293T Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
All lanes: Anti-RAB9A(ASA-B1603) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: Mouse Brain Tissue Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: PC12 Whole Cell Lysate at 40ug
Lane 5: NIH Whole Cell Lysate at 40ug
Lane 6: A431 Whole Cell Lysate at 40ug
Lane 7: 293T Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
