Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| tumor necrosis factor receptor superfamily, member 11a, NFKB activator | Polyclonal | IgG | Rabbit | Mouse | ELISA, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E. coli-derived mouse RANK recombinant protein (Position: C35-S214). Mouse RANK shares 95.6% amino acid (aa) sequence identity with rat RANK. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TNFRSF11A |
Protein |
Tumor necrosis factor receptor superfamily member 11A |
Uniprot ID |
O35305 |
Function |
Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. |
Tissue Specificity |
Ubiquitous expression with high levels in trabecular bone, thymus, small intestine, lung, brain and kidney. Weakly expressed in spleen and bone marrow. |
Sub-cellular localization |
Cell membrane ; Single-pass type I membrane protein . |
Sequence Similarities |
|
Aliases |
Activator of NFKB antibody|CD 265 antibody|CD265 antibody|CD265 antigen antibody|EOF antibody|FEO antibody|LOH18CR1 antibody|Loss of heterozygosity 18 chromosomal region 1 antibody|mRANK antibody|NFKB activator antibody| ODFR antibody|OFE antibody|OPTB7 antibody| Osteoclast differentiation factor receptor antibody|OSTS antibody|PDB 2 antibody|PDB2 antibody|RANK antibody| Receptor activator of NF KB antibody|Receptor activator of NF-KB antibody| receptor activator of nuclear factor kappa B antibody|TNFRSF 11A antibody| TNFRSF11A antibody|TNFSF11 antibody|TNR11_HUMAN antibody|TRANCE R antibody|TRANCER antibody|Tumor necrosis factor receptor superfamily member 11a NFKB activator antibody| Tumor necrosis factor receptor superfamily member 11a activator of NFKB antibody|Tumor Necrosis Factor Receptor Superfamily Member 11a antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio’s ECL kit |
| ELISA | 0.1-0.5μg/ml | Mouse | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- RANK antibody, ASA-B1610, Western blotting
All lanes: Anti RANK (ASA-B1610) at 0.5ug/ml
WB: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 66KD
Observed bind size: 100KD
All lanes: Anti RANK (ASA-B1610) at 0.5ug/ml
WB: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 66KD
Observed bind size: 100KD
