Description
Data Sheet
| Amino acid sequence | EKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID. |
| Biological Activity | The activity is determined by a dose-dependent stimulation of IL-8 production in human PBMC and is typically less than 100ng/ml, corresponding to a specific activity of 10,000 Units/mg. |
| Description | sRANKL Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 175 amino acids and having a molecular mass of 19.7kDa. |
| Expression host | Escherichia Coli. |
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Sodium phosphate, pH-7.5. |
| Protein Background | RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. |
| Purity | Greater than 95.0% as determined by analysis by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| References | 1. Title:Cathepsin K Expression and Activity in Canine Osteosarcoma.Publication: Article first published online: 15 DEC 2011 DOI:10.1111/j.1939-1676.2011.00834.x Copyright © 2011 by the American College of Veterinary Internal Medicine.Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1939-1676.2011.00834.x/full2.Title: Grape Seed Procyanidin Reversal of P-glycoprotein Associated Multi-Drug Resistance via Down-regulation of NF-?B and MAPK/ERK Mediated YB-1 Activity in A2780/T CellsPublication: DOI: 10.1371/journal.pone.0071071, August 15, 2013Link: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0071071#pone-0071071-g005 |
| Solubility | It is recommended to reconstitute the lyophilized sRANKL in sterile 18M-cm H2O at a concentration of 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | Soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf, hRANKL2. |
