Description
Assay Range
25 – 800 µg/ml
Sensitivity
5.0 µg/ml
Assay Principle
Sandwich ELISA
Storage
Store at 2 – 8â°C
Sample Type
Serum, plasma, body fluids, tissus lysates
Size
96T
Sample Volume
50 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Rat C3a standards (ready to use): 0.5ml/vial x 6.
2. One 96 well plate precoated with anti-Rat C3a antibody.
3. Sample diluent buffer: 6 ml x 1.
4. HRP conjugated detection antibody: 10 ml x 1.
5. Closure plate membrane: 2.
6. 20 x Wash buffer: 25 ml x 1.
7. TMB color developing agent A: 6 ml x 1.
8. TMB color developing agent B: 6 ml x 1.
9. TMB stop solution: 6 ml x 1.
For protocols and availability questions, please fill out our contact us form and make sure to include your company name, business email, and phone number. We will contact you within 24 hours.
Different lot may vary, please follow the instruction coming with the kit.
