Description
Kit Components:
1. Rat Hepcidin standards (ready to use): 0.5ml/vial x 7.
2. One 96 well plate precoated with anti-Rat Hepcidin antibody.
3. Sample diluent buffer: 12 ml x 2
4. HRP conjugated Rat Hepcidin
5. Closure plate membrane: 2.
6. 20 x Wash buffer: 25 ml x 1.
7. TMB color developing agent A: 6 ml x 1.
8. TMB color developing agent B: 6 ml x 1.
9. TMB stop solution: 6 ml x 1.
For protocols and availability questions, please fill out our contact us form and make sure to include your company name, business email, and phone number. We will contact you within 24 hours.
Different lot may vary, please follow the instruction coming with the kit.
PRODUCT CITATIONS
1. Lipopolysaccharides Upregulate Hepcidin in Neuron via Microglia and the IL-6/STAT3 Signaling Pathway
Qian ZM, He X, Liang T, Wu KC, Yan YC, Lu LN, Yang G, Luo QQ, Yung WH, Ke Y
Mol Neurobiol. 2014 Dec;50(3):811-20
Sample: Brain lysate
Du F, Qian ZM, Luo Q, Yung WH, Ke Y
Mol Neurobiol. 2014 Aug 13
Sample: Brain lysate
Data Sheet
| Alternate Names | HAMP; HEPC; hepcidin antimicrobial peptide; Hepcidin; HEPCPutative liver tumor regressor; HFE2B; HFE2Bhepcidin; LEAP1; LEAP-1; LEAP1PLTR; Liver-expressed antimicrobial peptide 1; PLTR |
| Amino Acid Sequenc | DTNFPICLFCCKCCKNSSCGLCCIT |
| Assay Range | 0.37 – 90 ng/ml |
| Assay Time | 90 minutes |
| Assay Type | Competitive ELISA |
| Dilution Factors | undiluted for normal plasma, serum |
| Gene ID | 84604 |
| Kit Type | Colorimetric ELISA |
| Precision | Intra-Assay, 4.8%; Inter-Assay 10.1% |
| Recovery | 89% to 112% |
| Sample Type | Plasma, Serum, tissue lysates, cell culture medium |
| Sample Volume | 100 ul |
| Sensitivity | 0.1 ng/ml |
| Specificity | recognizes both native and correctly folded recombinant Hepcidin |
| Storage | Storage of components varies. See protocol for specific instructions. |
