Rat Hypoxia Inducible Factor 1 Alpha HIF1a ELISA Kit

1. Rat HIF-1αstandard:  

20 ng/vial -2.

2. One 96-well plate pre-coated with anti-Rat HIF-1αAb:                         

1.

3. Sample  diluent buffer:                                                                                        

12 mL – 1.

4. Detection  antibody:

130 µL, dilution 1:100.

5. Streptavidin-HRP: 

130 µL, dilution 1:100.

6. Antibody diluent buffer: 

12 ml.

7. Streptavidin-HRP diluent buffer: 

12 ml.

8. TMB developing agent: 

10 ml.

9. Stop solution:

10 ml.

10. 20 – Wash Solution: 

25 ml.