Description
Assay Range
62.5 – 4000 pg/mL
Sensitivity
30 pg/mL
Assay Principle
Sandwich ELISA
Storage
Store at 2 – 8â°C
Sample Type
Serum, plasma, body fluids, tissus lysates
Size
96T/48T
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant Rat TPO standard: 8ng/vial x 1.
2. One 96-well plate precoated with anti-Rat TPO antibody.
3. Assay diluent buffer: 6 ml
4. HRP conjugated anti-Rat TPO antibody: 10 ml.
5. Sample diluention buffer: 10mL x 2.
6. 20 x Wash buffer: 25 ml.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 10 ml.
Background
Thrombopoietin is a glycoprotein hormone produced mainly by the liver and thekidney that regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets.
