Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| reticulon 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Reticulon 1(121-136aa TYFTGILQKENGHVTI), different from the related mouse and rat sequences by three amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
RTN1 |
Protein |
Reticulon-1 |
Uniprot ID |
Q16799 |
Function |
May be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. |
Tissue Specificity |
Expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. |
Sub-cellular localization |
Endoplasmic reticulum membrane; Multi-pass membrane protein. |
Sequence Similarities |
|
Aliases |
Neuroendocrine specific protein antibody|Neuroendocrine-specific protein antibody|NSP antibody|Reticulon 1 antibody|Reticulon-1 antibody|RTN 1 antibody|RTN1 antibody|RTN1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Reticulon 1 antibody, ASA-B1622, All Western blotting
All lanes: Anti-RTN1(ASA-B1622) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 40ug
Predicted bind size: 84KD
Observed bind size: 84KD
All lanes: Anti-RTN1(ASA-B1622) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 40ug
Predicted bind size: 84KD
Observed bind size: 84KD
