Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| ribonuclease, RNase A family, 1(pancreatic) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Ribonuclease A(72-90aa NTFVHEPLVDVQNVCFQEK). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
RNASE1 |
Protein |
Ribonuclease pancreatic |
Uniprot ID |
P07998 |
Function |
Endonuclease that catalyzes the cleavage of RNA on the 3′ side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA. |
Tissue Specificity |
Pancreas and other tissues and body fluids (indicating it may have other physiological functions besides its role in digestion). |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
|
Aliases |
HP-RNase antibody|Pancreatic ribonuclease antibody|Rib 1 antibody|RIB-1 antibody|Rib1 antibody|Ribonuclease 1 antibody|Ribonuclease A antibody|Ribonuclease a family 1 antibody|Ribonuclease pancreatic antibody|Ribonuclease RNase A family 1 antibody|Ribonuclease RNase A family 1 pancreatic antibody|RNAS1_HUMAN antibody|RNase 1 antibody|RNAse A antibody|RNase UpI-1 antibody|Rnase1 antibody|RNAseA antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Ribonuclease A antibody, ASA-B1628, Western blotting
All lanes: Anti Ribonuclease A (ASA-B1628) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: SW620 Whole Cell Lysate at 40ug
Lane 9: CEM Whole Cell Lysat
All lanes: Anti Ribonuclease A (ASA-B1628) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: SW620 Whole Cell Lysate at 40ug
Lane 9: CEM Whole Cell Lysat
