Description
Data Sheet
| Amino acid sequence | MKHHHHHHAS GSELETAMET LINVFHAHSG KEGDKYKLSK KELKELLQTE LSGFLDAQKD VDAVDKVMKE LDENGDGEVD FQEYVVLVAA LTVACNNFFW ENS. |
| Description | S100A1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 103 amino acids which include a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 11.66 kDa. S100A1 Human Recombinant is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The S100A1 protein was lyophilized from 0.4um filtered solution at a concentration of 0.5mg/ml containing 20mM Tris pH-7.5 and 50mM NaCl. |
| Protein Background | S100A1 is a member of the S100 family of calcium binding proteins with EF-hand type Ca+2 binding motive. S100A1 (Calcium Binding Protein A1) is involved in the activation of sarcoplasmatic calcium release and the regulation of intermediate filament polymerization. S100A1 may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of S100A1 has been implicated in cardiomyopathies.S100 proteins are localized either in the cytoplasm or the nucleus of a wide range of cells. There are at least 13 members in the S100 gene family, which are located as a cluster on chromosome 1q21. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Reagent Appearance | White lyophilized (freeze-dried) powder. |
| Solubility | Add deionized water to a working concentration approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture. |
| Stability | Lyophilized S100A1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution S100A1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | Protein S100-A1, S100 calcium-binding protein A1, S-100 protein alpha subunit, S-100 protein alpha chain, S100A1, S100A, S100, S100-alpha, S100-A1. |
