Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MHRVGQAGLQ LLTSSDPPAL DSQSAGITGA DANFSVWIKR CQEAQNGSES EVWTHQSKIK YDWYQTESQVVITLMIKNVQ KNDVNVEFSE KELSALVKLP SGEDYNLKLE LLHPIIPEQS TFKVLSTKIE IKLKKPEAVR WEKLEGQGDV PTPKQFVADVKNLYPSSSPY TRNWDKLVGE IKEEEKNEKL EGDAALNRLF QQIYSDGSDE VKRAMNKSFM ESGGTVLSTN WSDVGKRKVE INPPDDMEWKKY |
| Description | SGT1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (115-365.a.a) and having a molecular mass of 30.7kDa. SGT1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The SGT1 protein solution (0.5mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT and 20% glycerol. |
| Protein Background | SUGT1 is homolog to yeast protein SGT1, a regulator of the cell cycle which is vital for G1/S and G2/M transitions. SUGT1 holds a CS domain, a SGS domain, a p23 domain and three tetratricopeptide repeats (TPR). SUGT1 protein associates with Skp1 p19 and CUL-1, subunits of the SCF ubiquitin ligase complex, and is believed to take a part in protein degradation. Furthermore, SUGT1 is essential for the kinetochores assembly, and has a role as a co-chaperone for HSP90. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles. |
| Synonyms | SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae), SGT1, Putative 40-6-3 protein, SGT1B protein. |
