Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| SRY(sex determining region Y)-box 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human SOX2(171-183aa YSMMQDQLGYPQH), different from the related mouse and rat sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SOX2 |
Protein |
Transcription factor SOX-2 |
Uniprot ID |
P48431 |
Function |
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Contains 1 HMG box DNA-binding domain. |
Aliases |
ANOP3 antibody|cb236 antibody|Delta EF2a antibody|lcc antibody|MCOPS3 antibody|MGC148683 antibody|MGC2413 antibody|RGD1565646 antibody|Sex determining region Y box 2 antibody|SOX 2 antibody|Sox2 antibody|SRY(sex determining region Y) box 2 antibody|SRY box containing gene 2 antibody|SRY related HMG box 2 antibody|SRY related HMG box gene 2 antibody|SRY-box 2 antibody|Transcription factor SOX 2 antibody|Transcription factor SOX-2 antibody|ysb antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SOX2 antibody, ASA-B1759, IHC(P)
IHC(P): Rat Lung Tissue
IHC(P): Rat Lung Tissue
Anti-SOX2 antibody, ASA-B1759, IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti-SOX2 antibody, ASA-B1759, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
