Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| spectrin, alpha, erythrocytic 1(elliptocytosis 2); spectrin, beta, erythrocytic |
Monoclonal |
IgG |
Mouse |
Human |
WB |
Ascites |
Immunogen
|
| Human erythrocyte spectrin. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
SPTA1; SPTB |
Protein
|
Spectrin alpha chain, erythrocytic 1; Spectrin beta chain, erythrocytic |
Uniprot ID
|
Human(P02549), Mouse(P08032), Rat(D4A678), Human(P11277), Mouse(P15508), Rat(Q6XDA0) |
Function
|
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. |
Tissue Specificity
|
|
Sub-cellular localization
|
Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. |
Sequence Similarities
|
Belongs to the spectrin family. |
Aliases
|
EL2|HPP|HS3|SPH3|SPTA|EL3|HS2|SPH2|HSPTB1|Erythroid alpha-spectrin |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
2-4μg/ml |
Human |
AssaySolutio’s ECL kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
