Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| splicing factor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human splicing factor 1(11-30aa DFPSKKRKRSRWNQDTMEQK), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SF1 |
Protein |
Splicing factor 1 |
Uniprot ID |
Q15637 |
Function |
Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5′-UACUAAC-3′ of the pre-mRNA. May act as transcription repressor. |
Tissue Specificity |
Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart. |
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the BBP/SF1 family. |
Aliases |
BBP antibody|D11S636 antibody|Mammalian branch point-binding protein mBBP antibody|OTTHUMP00000069749 antibody|OTTHUMP00000069750 antibody|OTTHUMP00000069752 antibody|splicing factor 1 antibody|Transcription factor ZFM1 antibody|ZFM1 antibody|Zinc finger gene in MEN1 locus antibody|Zinc finger protein 162 antibody|ZNF162 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-splicing factor 1 antibody, ASA-B1775, Western blotting
All lanes: Anti splicing factor 1 (ASA-B1775) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Lane 6: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 52KD
Observed bind size: 70KD
All lanes: Anti splicing factor 1 (ASA-B1775) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Lane 6: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 52KD
Observed bind size: 70KD
Anti-splicing factor 1 antibody, ASA-B1775, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-splicing factor 1 antibody, ASA-B1775, ICC
ICC: HELA Cell
ICC: HELA Cell
