Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| signal sequence receptor, gamma(translocon-associated protein gamma) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human SSR3(8-23aa KQQSEEDLLLQDFSRN), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SSR3 |
Protein |
Translocon-associated protein subunit gamma(TRAP-gamma) |
Uniprot ID |
Q9UNL2 |
Function |
TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. |
Tissue Specificity |
|
Sub-cellular localization |
Endoplasmic reticulum membrane; Multi-pass membrane protein. |
Sequence Similarities |
|
Aliases |
Signal sequence receptor subunit gamma antibody|SSR-gamma antibody|Ssr3 antibody|SSRG_HUMAN antibody|Translocon-associated protein subunit gamma antibody|TRAP-gamma antibody|TRAPG antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SSR3 antibody, ASA-B1784, Western blotting
All lanes: Anti SSR3 (ASA-B1784) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: A431 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: U87 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
All lanes: Anti SSR3 (ASA-B1784) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: A431 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: U87 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
Anti-SSR3 antibody, ASA-B1784, IHC(P)
IHC(P): Human Prostatic Cancer Tissue
IHC(P): Human Prostatic Cancer Tissue
Anti-SSR3 antibody, ASA-B1784, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
