Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| stanniocalcin 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Stanniocalcin 2(287-302aa SSEWEDEQSEYSDIRR), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
STC2 |
Protein |
Stanniocalcin-2 |
Uniprot ID |
O76061 |
Function |
Has an anti-hypocalcemic action on calcium and phosphate homeostasis. |
Tissue Specificity |
Expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leukocytes. |
Sub-cellular localization |
Secreted . |
Sequence Similarities |
|
Aliases |
Stanniocalcin related protein antibody|Stanniocalcin-2 antibody|Stanniocalcin-related protein antibody|Stanniocalcin2 antibody|STC 2 antibody|STC related protein antibody|STC-2 antibody|STC-related protein antibody|STC2 antibody|STC2_HUMAN antibody|STCRP antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Stanniocalcin 2 antibody, ASA-B1788, Western blotting
All lanes: Anti STC2 (ASA-B1788) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: Rat Kidney Tissue Lysate at 50ug
Lane 4: Rat Intestine Tissue Lysate at 50ug
Predicted bind size: 23KD
Observed bind size: 23KD
All lanes: Anti STC2 (ASA-B1788) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: Rat Kidney Tissue Lysate at 50ug
Lane 4: Rat Intestine Tissue Lysate at 50ug
Predicted bind size: 23KD
Observed bind size: 23KD
