Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| signal transducer and activator of transcription 5B | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human STAT5b(767-787aa RRVEELLGRPMDSQWIPHAQS), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
STAT5B |
Protein |
Signal transducer and activator of transcription 5B |
Uniprot ID |
P51692 |
Function |
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm . Nucleus . Note: Translocated into the nucleus in response to phosphorylation. |
Sequence Similarities |
Belongs to the transcription factor STAT family. |
Aliases |
Signal transducer and activator of transcription 5B antibody|STA5B_HUMAN antibody|STAT5 antibody|Stat5b antibody|Transcription factor STAT5B antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-STAT5b antibody, ASA-B1798, Western blotting
Lane 1: Rat Intestine Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: MM231 Cell Lysate
Lane 6: COLO320 Cell Lysate
Lane 1: Rat Intestine Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: MM231 Cell Lysate
Lane 6: COLO320 Cell Lysate
Anti-STAT5b antibody, ASA-B1798, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
