Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cystatin B(stefin B) | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of mouse Stefin B(62-77aa DKCVHLRVFQPLPHEN), different from the related rat sequence by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CSTB |
Protein |
Cystatin-B |
Uniprot ID |
Q62426 |
Function |
This is an intracellular thiol proteinase inhibitor. |
Tissue Specificity |
Widely expressed. Highest expression in heart, liver and kidney. Lower levels in brain, lung and skeletal muscle. Lowest levels in spleen and testis. |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
|
Aliases |
CHROW21 antibody|CPI B antibody|CPI-B antibody|CST6 antibody|CSTB antibody|Cystatin B antibody|Cystatin-B antibody|CYTB antibody|CYTB_HUMAN antibody|EPM1 antibody|EPM1A antibody|Liver thiol proteinase inhibitor antibody|PME antibody|Stefin-B antibody|STFB antibody|ULD antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- StefinB antibody, ASA-B1804, Western blotting
All lanes: Anti StefinB (ASA-B1804) at 0.5ug/ml
WB: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 11KD
Observed bind size: 11KD
All lanes: Anti StefinB (ASA-B1804) at 0.5ug/ml
WB: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 11KD
Observed bind size: 11KD
