Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| superoxide dismutase 1, soluble | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of Human Superoxide Dismutase 1(113-129aa IIGRTLVVHEKADDLGK), different from the mouse sequence by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SOD1 |
Protein |
Superoxide dismutase[Cu-Zn] |
Uniprot ID |
P00441 |
Function |
Destroys radicals which are normally produced within the cells and which are toxic to biological systems. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. Nucleus. Note: Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. |
Sequence Similarities |
Belongs to the Cu-Zn superoxide dismutase family. |
Aliases |
ALS 1 antibody|ALS antibody|ALS1 antibody|Amyotrophic lateral sclerosis 1 adult antibody|Amyotrophic lateral sclerosis 1 antibody|Cu/Zn SOD antibody|Cu/Zn superoxide dismutase antibody|Homodimer antibody|hSod1 antibody|Indophenoloxidase A antibody|IPOA antibody|Mn superoxide dismutase antibody|SOD 1 antibody|SOD antibody|SOD soluble antibody|SOD1 antibody|SODC antibody|SODC_HUMAN antibody|Superoxide dismutase[Cu Zn] antibody|Superoxide dismutase[Cu-Zn] antibody|Superoxide dismutase 1 antibody|Superoxide dismutase 1 soluble antibody|Superoxide dismutase antibody|Superoxide dismutase Cu Zn antibody|Superoxide dismutase cystolic antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse, Rat Human | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Superoxide Dismutase 1 antibody, ASA-B1811, Western blotting
Lane 1: COLO320 Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 1: COLO320 Cell Lysate
Lane 2: SMMC Cell Lysate
Anti-Superoxide Dismutase 1 antibody, ASA-B1811, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
