Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| surfactant protein A1 | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse Surfactant Protein A(233-248aa WNDKGCLQYRLAICEF), identical to the related rat sequence. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SFTPA1 |
Protein |
Pulmonary surfactant-associated protein A1(PSP-A/PSPA/SP-A/SP-A1) |
Uniprot ID |
P35242 |
Function |
In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film. |
Sequence Similarities |
Belongs to the SFTPA family. |
Aliases |
35 kDa pulmonary surfactant-associated protein antibody|Alveolar proteinosis protein antibody|COLEC4 antibody|Collectin 4 antibody|PSAP antibody|PSPA antibody|Pulmonary surfactant apoprotein antibody|Pulmonary surfactant-associated protein A1 antibody|SFTP1 antibody|SFTPA antibody|SFTPA1 antibody|SFTPA1B antibody|SP A antibody|SP A1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Surfactant Protein A antibody, ASA-B1813, Western blotting
Lane 1: Mouse Lung Tissue Lysate
Lane 2: Mouse Lung Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Lane 4: Rat Lung Tissue Lysate
Lane 1: Mouse Lung Tissue Lysate
Lane 2: Mouse Lung Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Lane 4: Rat Lung Tissue Lysate
Anti-Surfactant Protein A antibody, ASA-B1813, IHC(P)
IHC(P): Rat Lung Tissue
IHC(P): Rat Lung Tissue
