Description
Data Sheet
| Applications | VAMP2 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000. |
| Bilogical Activity | BCA – to determine content of the protein. SDS PAGE – to determine purity of the protein. |
| Clone | P3E5AT. |
| Ig Subclass | Mouse IgG1 heavy chain and light chain. |
| Immunogen | Anti-human VAMP2 mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human VAMP2 amino acids 1-89 purified from E. coli. |
| Protein Background | Synaptobrevin 2 which is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein (residues 1-89) forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25. |
| Protein formulation | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. |
| Purification Method | VAMP2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. |
| Stability / Shelf Life | 12 months at -20°C. 1 month at 4°C. |
| Storage Procedures | For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. |
| Synonyms | Vesicle-associated membrane protein 2, SYB2, VAMP-2, Synaptobrevin-2, VAMP2, FLJ11460. |
| Type | Mouse Anti Human Monoclonal. |
