Description
Data Sheet
| Antibody Properties | Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Applications | VAMP3 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000. |
| Clone | Pk2A2AT. |
| Ig Subclass | Mouse IgG2b heavy chain and light chain. |
| Immunogen | Anti-human VAMP3 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human VAMP3 amino acids 1-77 purified from E. coli. |
| Protein Background | VAMP3 is present in recycling endosomes and endosome-derived vesicles. VAMP3 has been implicated in recycling of transferrin receptors to the plasma membrane, secretion of alpha-granules in platelets, recycling of T-cell receptors to the immunological synapses, and membrane trafficking during cell migration. VAMP-3 is present in human platelets and necessary for granule secretion. Synaptobrevins are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP3 high homology to other VAMPs in its broad tissue distribution and subcellular localization is shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. |
| Protein content | By direct ELISA, 1:10,000 dilution will yield 0.4 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
| Protein formulation | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. |
| Purification Method | VAMP3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. |
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Stability / Shelf Life | 12 months at -20°C. 1 month at 4°C. |
| Storage Procedures | For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. |
| Synonyms | VAMP3, VAMP-3, Cellubrevin, Vesicle-Associated Membrane Protein 3, Synaptobrevin-3, CEB, SYB3. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |
| Type | Mouse Anti Human Monoclonal. |
