Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| synaptophysin | Monoclonal | IgG | Mouse | Human, Rat | IHC-P, IHC-F, WB | Ascites |
Immunogen |
||||||
| Rat retina synaptosome. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration |
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SYP |
Protein |
Synaptophysin |
Uniprot ID |
Human(P08247), Mouse(Q62277), Rat(P07825) |
Function |
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity). |
Tissue Specificity |
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. |
Sub-cellular localization |
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome. |
Sequence Similarities |
Belongs to the synaptophysin/synaptobrevin family. |
Aliases |
MRXSYP|Major synaptic vesicle protein p38 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.5-1μg/ml | Human, rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 1-2μg/ml | Human, rat | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 1-2μg/ml | Human, rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
-ASA-B1817-1.jpg)
Anti-Synaptophysin antibody (monoclonal), ASA-B1817, Western blotting
WB: Rat Brain Tissue Lysate
WB: Rat Brain Tissue Lysate
-ASA-B1817-2.jpg)
Anti-Synaptophysin antibody (monoclonal), ASA-B1817, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
-ASA-B1817-3.jpg)
Anti-Synaptophysin antibody (monoclonal), ASA-B1817, IHC(P)
IHC(P): Human Glioma Tissue
IHC(P): Human Glioma Tissue
