Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| syndecan 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse Syndecan 3(45-60aa AQRWRNENFERPVDLE), identical to the related rat sequence, and different from the related human sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SDC3 |
Protein |
Syndecan-3 |
Uniprot ID |
Q64519 |
Function |
Cell surface proteoglycan that may bear heparan sulfate. May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism (By similarity). |
Tissue Specificity |
|
Sub-cellular localization |
Membrane; Single-pass type I membrane protein. |
Sequence Similarities |
|
Aliases |
N-syndecan antibody|Sdc3 antibody|SDC3_HUMAN antibody|SDCN antibody|SYND3 antibody|syndecan proteoglycan 3 antibody|Syndecan-3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Syndecan 3 antibody, ASA-B1823, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti-Syndecan 3 antibody, ASA-B1823, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-Syndecan 3 antibody, ASA-B1823, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: 293T Cell Lysate
Lane 3: PC-12 Cell Lysate
Lane 4: NRK Cell Lysate
Lane 5: HT1080 Cell Lysate
Lane 1: U87 Cell Lysate
Lane 2: 293T Cell Lysate
Lane 3: PC-12 Cell Lysate
Lane 4: NRK Cell Lysate
Lane 5: HT1080 Cell Lysate
