Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MAMSLPGSRR TSAGSRRRTS PPVSVRDAYG TSSLSSSSNS GSYKGSDSSP TPRRSMKYTL CSDNHGIKPPTPEQYLTPLQ QKEVCIRHLK ARLKDTQDRL QDRDTEIDDL KTQLSRMQED WIEEECHRVE AQLALKEARK EIKQLKQVID TVKNNLIDKDKGLQKYFVDI NIQNKKLETL LHSMEVAQNG MAKEDGTGES AGGSPARSLT RSSTYTKLSD PAVCGDRQPG DPSSGSAEDG ADSGFAAADDTLSRTDALEA SSLLSSGVDC GTEETSLHSS FGLGPRFPAS NTYEKLLCGM EAGVQASCMQ ERAIQTDFVQ YQPDLDTILE KVTQAQVCGTDPESGDRCPE LDAHPSGPRD PNSAVVVTVG DELEAPEPIT RGPTPQRPGA NPNPGQSVSV VCPMEEEEEA AVAEKEPKSY WSRH. |
| Antibody Properties | Reconstitute with 0.5ml H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Description | SNPH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 444 amino acids (1-424) and having a molecular mass of 48.2 kDa.The SNPH is fused to 20 amino acid His-Tag at N-terminus and purified by standard chromatography techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The Syntaphilin protein solution contains 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol. |
| Protein Background | Syntaxin-1, synaptobrevin, and SNAP25 cooperate to form the SNARE complex, which is needed for synaptic vesicle docking and fusion SNPH is a neuron-specific protein originally characterized as a binding partner of syntaxin-1. SNPH participates with SNAP25 for the binding to Syntaxin-1 and prevents the construction of the SNARE core complex, thus manageling free syntaxin-1 availability for the assembly of the SNARE complex and potentially regulating synaptic vesicle exocytosis. Expression Syntaphilin appears to be brain-specific. SNPH is an inhibitor of both SNARE-based fusion and dynamin-mediated endocytosis. |
| Protein content | In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson ImmunoResearch Laboratories) 1:10,000 dilution will yield 0.7 O.D within 10 minutes. |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Solubility | This antibody will bind very well to protein A in a buffer (PBS) containing high salt concentration (3M Nacl). |
| Stability | SNPH although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | KIAA0374, MGC46096, bA314N13.5, SNPH, Syntaphilin. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |
