Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| syntaxin 1A (brain) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Syntaxin 1a(1-28aa MKDRTQELRTAKDSDDDDDVAVTVDRDR), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
STX1A |
Protein |
Syntaxin-1A |
Uniprot ID |
Q16623 |
Function |
Plays a role in hormone and neurotransmitter exocytosis (By similarity). Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)- regulation of exocytosis acrosomal reaction in sperm. |
Tissue Specificity |
Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain. |
Sub-cellular localization |
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass type IV membrane protein . Cell junction, synapse, synaptosome . Cell membrane. |
Sequence Similarities |
Belongs to the syntaxin family. |
Aliases |
EPIM antibody|Epimorphin antibody|EPM antibody|FP17469 antibody|HPC 1 antibody|Neuron specific antigen HPC 1 antibody|Neuron specific antigen HPC1 antibody|neuron-specific antigen HPC 1 antibody|P35 1 antibody|P35 1 antibody| STX 1 antibody|STX 1A antibody|STX1 antibody|STX1 antibody|STX1A antibody| Stx1b antibody|STX1B_HUMAN antibody|STX1B1 antibody|STX1B2 antibody|STX2 antibody|STX2A antibody|STX2B antibody|STX2C antibody|STX3 antibody|STX3A antibody|Syntaxin 1A antibody|Syntaxin 1A brain antibody|Syntaxin 1B antibody| Syntaxin 1B1 antibody|Syntaxin 1B2 antibody|Syntaxin 2 antibody|Syntaxin 3 antibody|Syntaxin 3A antibody|Syntaxin-1B antibody|Syntaxin-1B1 antibody| Syntaxin-1B2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Syntaxin 1a antibody, ASA-B1825, Western blotting
All lanes: Anti Syntaxin 1a (ASA-B1825) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 37KD
All lanes: Anti Syntaxin 1a (ASA-B1825) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 37KD
