Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| talin 1; talin 2 |
Monoclonal |
IgG |
Mouse |
Bovine, Human |
WB |
Ascites |
Immunogen
|
| Purified chicken gizzard talin. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
TLN1; TLN2 |
Protein
|
Talin-1; Talin-2 |
Uniprot ID
|
Human(Q9Y490), Mouse(P26039), Rat(Q498D4); Human(Q9Y4G6), Mouse(Q71LX4), Rat(D3ZA84) |
Function
|
Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts (By similarity). |
Tissue Specificity
|
|
Sub-cellular localization
|
Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell surface . Cell junction, focal adhesion . Note: Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity). |
Sequence Similarities
|
Contains 1 FERM domain. |
Aliases
|
TLN|KIAA1027|ILWEQ|TALIN |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
2-4μg/ml |
Human, mouse, rat, chicken |
AssaySolutio’s ECL kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
