Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| transglutaminase 1(K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human TGM1(498-511aa NSDKVYWQRQDDG), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TGM1 |
Protein |
Protein-glutamine gamma-glutamyltransferase K |
Uniprot ID |
P22735 |
Function |
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross- linking epidermal proteins during formation of the stratum corneum. |
Tissue Specificity |
|
Sub-cellular localization |
Membrane; Lipid-anchor. |
Sequence Similarities |
Belongs to the transglutaminase superfamily. Transglutaminase family. |
Aliases |
ARCI1 antibody|Epidermal TGase antibody|ICR2 antibody|KTG antibody|LI antibody|LI1 antibody|Protein glutamine gamma glutamyltransferase K antibody|Protein-glutamine gamma-glutamyltransferase K antibody|TG(K) antibody|TGase 1 antibody|TGASE antibody|TGase K antibody|TGase-1 antibody|TGK antibody|TGM1 antibody|TGM1_HUMAN antibody|Transglutaminase 1(K polypeptide epidermal type I, protein glutamine gamma glutamyltransferase) antibody|Transglutaminase 1 antibody|Transglutaminase K antibody|Transglutaminase, keratinocyte antibody|Transglutaminase-1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TGM1 antibody, ASA-B1853, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: COLO320 Cell Lysate
