Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| TIA1 cytotoxic granule-associated RNA binding protein | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human TIA1(98-110aa STQRSQDHFHVFV), identical to the related mouse sequence. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TIA1 |
Protein |
Nucleolysin TIA-1 isoform p40 |
Uniprot ID |
P31483 |
Function |
Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3′ untranslated regions (3′ UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasmic granule. Nucleus. Note: Accumulates in cytoplasmic stress granules (SG) following cellular damage. |
Sequence Similarities |
|
Aliases |
Cytotoxic granule associated RNA binding protein 1 antibody|Cytotoxic granule associated RNA binding protein antibody|mTIA-1 antibody|Nucleolysin TIA 1 isoform p40 antibody|Nucleolysin TIA-1 isoform p40 antibody|Nucleolysin TIA1 isoform p40 antibodyp40 TIA 1 antibody|p40-TIA-1(containing p15-TIA-1) antibody|p40-TIA-1 antibody|RNA binding protein TIA 1 antibody|RNA binding protein TIA1 antibody|RNA-binding protein TIA-1 antibody|T-cell-restricted intracellular antigen-1 antibody|TIA 1 antibody|TIA 1 cytotoxic granule associated RNA binding protein antibody|Tia antibody|TIA-1 antibody|TIA1 antibody|TIA1 cytotoxic granule associated RNA binding protein antibody|TIA1 cytotoxic granule associated RNA binding protein like 1 antibody|TIA1 protein antibody|TIA1_HUMAN antibody|TIAL1 antibody|TIAR antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TIA1 antibody, ASA-B1858, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: RAJI Cell Lysate
Lane 3: CEM Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: K562 Cell Lysate
Lane 1: JURKAT Cell Lysate
Lane 2: RAJI Cell Lysate
Lane 3: CEM Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: K562 Cell Lysate
