Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| tumor necrosis factor receptor superfamily, member 1B | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human TNF Receptor II(103-121aa DQVETQACTREQNRICTCR). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TNFRSF1B |
Protein |
Tumor necrosis factor receptor superfamily member 1B |
Uniprot ID |
P20333 |
Function |
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. |
Tissue Specificity |
|
Sub-cellular localization |
Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities |
Contains 4 TNFR-Cys repeats. |
Aliases |
CD 120b antibody|CD120b antibody|CD120b antigen antibody|Etanercept antibody|p75 antibody|p75TNFR antibody|p80 TNF alpha receptor antibody|p80 TNF-alpha receptor antibody|TBP-2 antibody|TBP2 antibody|TBPII antibody|TNF R II antibody|TNF R2 antibody|TNF R75 antibody|TNF-R2 antibody|TNF-RII antibody|TNFBR antibody|TNFR 2 antibody|TNFR-II antibody|TNFR2 antibody|TNFR75 antibody|TNFR80 antibody|TNFRII antibody|TNFRSF 1B antibody|TNFRSF1B antibody|TNR1B antibody|TNR1B_HUMAN antibody|Tumor necrosis factor beta receptor antibody|Tumor necrosis factor binding protein 2 antibody|Tumor necrosis factor receptor 2 antibody|Tumor necrosis factor receptor superfamily member 1B precursor antibody|Tumor necrosis factor receptor type II antibody|Tumor necrosis factor-binding protein 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TNF Receptor II antibody, ASA-B1887, Western blotting
Lane 1: MM453 Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 1: MM453 Cell Lysate
Lane 2: JURKAT Cell Lysate
Anti-TNF Receptor II antibody, ASA-B1887, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
