Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| TNF receptor-associated factor 1 | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human TRAF1(220-235aa HQSQLDRERILSLEQR), different from the related mouse sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TRAF1 |
Protein |
TNF receptor-associated factor 1 |
Uniprot ID |
Q13077 |
Function |
Adapter molecule that regulates the activation of NF- kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein- ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2. |
Tissue Specificity |
|
Sub-cellular localization |
|
Sequence Similarities |
Contains 1 MATH domain. |
Aliases |
4732496E14Rik antibody|EBI6 antibody|EBV induced protein 6 antibody|Epstein-Barr virus-induced protein 6 antibody|MGC:10353 antibody|Tnf receptor associated factor 1 antibody|TNF receptor-associated factor 1 antibody|TRAF 1 antibody|TRAF1 antibody|TRAF1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TRAF1 antibody, ASA-B1900, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human TRAF1, 30.6KD(162aa tag+ E174-E282)
Lane 1: Recombinant Human TRAF1 Protein 5ng
Lane 2: Recombinant Human TRAF1 Protein 2.5ng
Lane 3: Recombinant Human TRAF1 Protein 1.25ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human TRAF1, 30.6KD(162aa tag+ E174-E282)
Lane 1: Recombinant Human TRAF1 Protein 5ng
Lane 2: Recombinant Human TRAF1 Protein 2.5ng
Lane 3: Recombinant Human TRAF1 Protein 1.25ng
