Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| troponin T type 1(skeletal, slow); troponin T type 2(cardiac); troponin T type 3(skeletal, fast) |
Monoclonal |
IgG |
Mouse |
Chicken, Rabbit, Rat |
IHC-P, IHC-F, WB |
Ascites |
Immunogen
|
| Troponin T from rabbit skeletal muscle. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
TNNT1; TNNT2; TNNT3 |
Protein
|
Troponin T, slow skeletal muscle; Troponin T, cardiac muscle; Troponin T, fast skeletal muscle |
Uniprot ID
|
Human(P13805), Mouse(O88346), Rat(Q7TNB2), Human(P45379), Mouse(P50752), Rat(P50753), Human(P45378), Mouse(Q9QZ47), Rat(P09739) |
Function
|
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
Tissue Specificity
|
|
Sub-cellular localization
|
|
Sequence Similarities
|
Belongs to the troponin T family. |
Aliases
|
TNT|TNTF |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
2-4μg/ml |
Rat, rabbit, chicken. |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
4-8μg/ml |
Rat, rabbit, chicken. |
AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) |
4-8μg/ml |
Rat, rabbit, chicken. |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Troponin T antibody, ASA-B1928, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue